6CZ7
The arsenate respiratory reductase (Arr) complex from Shewanella sp. ANA-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 231.259, 85.639, 146.676 |
| Unit cell angles | 90.00, 127.84, 90.00 |
Refinement procedure
| Resolution | 46.910 - 1.620 |
| R-factor | 0.1376 |
| Rwork | 0.137 |
| R-free | 0.15470 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXD |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.910 | 1.678 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rpim | 0.031 | 0.320 |
| Number of reflections | 286352 | 28526 |
| <I/σ(I)> | 17.77 | 2.4 |
| Completeness [%] | 99.9 | 99.99 |
| Redundancy | 6.8 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 293 | 10 mg/ml protein, 15% PEG 2000 MME, 150 mM KSCN, 50 mM HEPES (adjusted to pH 7.5 with NaOH), microseeded; cryoprotected in 30% PEG 2000 MME, 100 mM KSCN, 1 M sodium formate, 50 mM HEPES (pH 7.5) |
