6CZ1
Crystal structure of ATPase domain of Human GRP78 bound to Ver155008
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-31 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97949 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.280, 74.920, 86.200 |
Unit cell angles | 90.00, 98.52, 90.00 |
Refinement procedure
Resolution | 24.600 - 1.680 |
R-factor | 0.189 |
Rwork | 0.187 |
R-free | 0.21800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5evz |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.21) |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 85.263 | 1.720 |
High resolution limit [Å] | 1.680 | 1.680 |
Rmeas | 0.073 | |
Rpim | 0.032 | |
Number of reflections | 79224 | 5822 |
<I/σ(I)> | 17.5 | |
Completeness [%] | 99.5 | 100 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 24-26% PEG3350, 0.1 M Tris-HCl, 0.2 M sodium chloride |