6CYO

Crystal structure of human UBE2A (RAD6A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-04
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.45863
Spacegroup name	P 21 21 21
Unit cell lengths	37.497, 51.350, 88.663
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.657 - 1.850
R-factor	0.2045
Rwork	0.204
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yb6
Data reduction software	XDS
Data scaling software	Aimless (0.5.21)
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.440	44.440	1.890
High resolution limit [Å]	1.850	9.060	1.850
Rmerge	0.064	0.040	1.692
Rmeas	0.067	0.042	1.802
Rpim	0.019	0.013	0.601
Number of reflections	15168	164	910
<I/σ(I)>	18.3
Completeness [%]	99.4	99.8	98.3
Redundancy	11.4	9.6	8.7
CC(1/2)	0.999	0.998	0.602

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	50 mM di-Sodium succinate pH 7.0 and 12-14% PEG 3350