6CY8

Crystal structure of FAD-dependent dehydrogenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 64 2 2
Unit cell lengths	104.365, 104.365, 235.671
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.195 - 2.729
R-factor	0.1937
Rwork	0.191
R-free	0.24060
RMSD bond length	0.008
RMSD bond angle	0.966
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.750
High resolution limit [Å]	2.700	2.700
Number of reflections	19969
<I/σ(I)>	12.52
Completeness [%]	95.9
Redundancy	11.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		279	Ammonium sulfate