6CY2
RNA octamer containing 2'-OMe, 4'Calpha-OMe U.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-17 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9183 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 31.500, 31.500, 85.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.400 - 1.400 |
R-factor | 0.185 |
Rwork | 0.184 |
R-free | 0.20510 |
Structure solution method | SAD |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | SHELXDE |
Refinement software | PHENIX (1.13_2992) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.400 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Rpim | 0.030 | 0.684 |
Number of reflections | 16014 | |
<I/σ(I)> | 11 | |
Completeness [%] | 98.7 | |
Redundancy | 13.5 | |
CC(1/2) | 0.997 | 0.529 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 1 mM RNA; 40 mM sodium cacodylate, 80 mM potassium chloride, 20 mM magnesium chloride, 12 mM spermine. Reservoir 40% MPD |