6CY1

Crystal structure of Signal recognition particle receptor FtsY from Elizabethkingia anophelis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2017-08-18
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 61
Unit cell lengths	70.800, 70.800, 229.050
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.850 - 1.900
R-factor	0.173
Rwork	0.172
R-free	0.19970
Structure solution method	MOLECULAR REPLACEMENT
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.850	41.850	1.950
High resolution limit [Å]	1.900	8.500	1.900
Rmerge	0.064	0.042	0.560
Rmeas	0.068	0.044	0.591
Total number of observations	491587
Number of reflections	51029	595	3801
<I/σ(I)>	21.02	45.5	4.01
Completeness [%]	100.0	98.3	100
Redundancy	9.633	8.973	9.6
CC(1/2)	0.999	0.999	0.907

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.7	290	tray 291453 B12: JCSG+ : 200 mM Potassium citrate tribasic, 20% (w/v) PEG 3350: Cryo = 20%EG : ElanA.00983.a.B1 at 20 mg/ml, puck drh8-9