6CXW
Synaptotagmin 1 C2A beta4 chimera with enhanced PIP2 binding function
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2016-01-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.283572 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 27.143, 45.105, 89.278 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.728 - 1.832 |
| R-factor | 0.1785 |
| Rwork | 0.175 |
| R-free | 0.21090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wee |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.716 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 31.730 | 31.730 | 1.870 |
| High resolution limit [Å] | 1.830 | 8.970 | 1.830 |
| Rmerge | 0.034 | 0.054 | 0.069 |
| Rmeas | 0.036 | 0.057 | 0.085 |
| Rpim | 0.011 | 0.018 | 0.048 |
| Total number of observations | 85169 | 865 | 1221 |
| Number of reflections | 9789 | 93 | 484 |
| <I/σ(I)> | 51.6 | 78.2 | 12.4 |
| Completeness [%] | 96.8 | 89.5 | 80.2 |
| Redundancy | 8.7 | 9.3 | 2.5 |
| CC(1/2) | 0.999 | 0.999 | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 300 | 50% PEG-4000, 0.4 mM Sodium acetate, 100 mM Tris |
