6CXB
Structure of N-truncated R1-type pyocin tail fiber at 1.7 angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 120 |
Detector technology | PIXEL |
Collection date | 2013-12-03 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.987 |
Spacegroup name | I 21 3 |
Unit cell lengths | 102.179, 102.179, 102.179 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.714 - 1.701 |
R-factor | 0.1611 |
Rwork | 0.159 |
R-free | 0.17660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ct8 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.788 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (2.6.0) |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.730 |
High resolution limit [Å] | 1.700 | 4.610 | 1.700 |
Rmerge | 0.074 | 0.026 | 0.919 |
Rmeas | 0.077 | 0.027 | |
Rpim | 0.025 | 0.008 | 0.536 |
Number of reflections | 19654 | 1053 | 981 |
<I/σ(I)> | 11.8 | ||
Completeness [%] | 100.0 | 100 | 99.8 |
Redundancy | 9.4 | 10.5 | 3.6 |
CC(1/2) | 0.999 | 0.520 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | Crystals were grown at a 1:1 protein (5 mg/mL) to precipitant (100mM Bicine pH 9.5, 20% PEG2k, 1mM CuSO4) ratio |