6CX4
V180A Mutant of Yeast PCNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-15 |
Detector | NOIR-1 |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 3 |
Unit cell lengths | 122.555, 122.555, 122.555 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.510 - 3.081 |
R-factor | 0.2473 |
Rwork | 0.246 |
R-free | 0.26660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1plq |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 24.510 | 24.510 | 3.190 |
High resolution limit [Å] | 3.080 | 6.600 | 3.080 |
Rmerge | 0.108 | 0.047 | 0.720 |
Rmeas | 0.116 | 0.051 | 0.765 |
Total number of observations | 99880 | ||
Number of reflections | 11585 | 1228 | 1150 |
<I/σ(I)> | 9.3 | 28.6 | 2 |
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 8.56 | 7.83 | 8.81 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 290.15 | 2.2M Ammonium Sulfate 0.2M Potassium Chloride 20% Glycerol |