6CX3
S179T Mutant of Yeast PCNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-04 |
Detector | NOIR-1 |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 3 |
Unit cell lengths | 123.756, 123.756, 123.756 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.750 - 3.101 |
R-factor | 0.2352 |
Rwork | 0.233 |
R-free | 0.26940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1plq |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 24.750 | 24.750 | 3.210 |
High resolution limit [Å] | 3.100 | 6.640 | 3.100 |
Rmerge | 0.142 | 0.045 | 0.752 |
Rmeas | 0.150 | 0.048 | 0.796 |
Total number of observations | 109371 | ||
Number of reflections | 11688 | 1232 | 1135 |
<I/σ(I)> | 8.2 | 32.2 | 2.1 |
Completeness [%] | 99.9 | 99.3 | 100 |
Redundancy | 9.29 | 8.52 | 9.32 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 290.15 | 2.2M Ammonium sulfate 0.2 Sodium Formate |