6CX0
Structure of AtTPC1 D376A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-21 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 88.470, 154.420, 218.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.000 - 3.501 |
R-factor | 0.3199 |
Rwork | 0.318 |
R-free | 0.35190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5dqq |
RMSD bond length | 0.003 |
RMSD bond angle | 0.687 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 4.000 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmeas | 0.239 | |
Number of reflections | 19231 | 6273 |
<I/σ(I)> | 8.08 | |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 13.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.3 | 292 | Sitting drop 2microliter drops crystchem 0.1 M glycine, pH 9.3, 50 mM potassium chloride, 1 mM calcium chloride, 35% PEG300 |