6CWX

Crystal structure of human ribonuclease P/MRP proteins Rpp20/Rpp25

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-29
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	I 4 3 2
Unit cell lengths	182.190, 182.190, 182.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.690 - 2.250
R-factor	0.2143
Rwork	0.213
R-free	0.23220
Structure solution method	SIRAS
RMSD bond length	0.012
RMSD bond angle	1.384
Data reduction software	iMOSFLM (7.2.1)
Data scaling software	Aimless (0.5.29)
Phasing software	autoSHARP (3.10.1)
Refinement software	REFMAC (5.8.0218)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.690	2.320
High resolution limit [Å]	2.250	2.250
Rmerge	0.083	0.495
Rmeas	0.087	0.523
Rpim	0.028	0.165
Number of reflections	24742	2265
<I/σ(I)>	16.4	4.7
Completeness [%]	100.0	100
Redundancy	9.7	9.8
CC(1/2)	0.999	0.924

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291.15	100 mM potassium formate, pH 7.5, 300 mM magnesium sulfate, 4.5% PEG 300, 4.5% PEG 400, 4.5% PEG 1000, 4.5% PEG 4000, and 4.5% PEG 8000