6CWK
Anti-RTA VHH antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 60.294, 60.294, 81.389 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.766 - 1.256 |
R-factor | 0.1562 |
Rwork | 0.155 |
R-free | 0.17550 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.023 |
RMSD bond angle | 1.862 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.280 |
High resolution limit [Å] | 1.256 | 1.260 |
Number of reflections | 41576 | |
<I/σ(I)> | 57.8 | |
Completeness [%] | 99.7 | |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 200 mM NaCl, 100 mM Na Acetate, 1.7 M Ammonium sulfate |