6CW5

Crystal structure of Ribokinase from Cryptococcus neoformans var. grubii serotype A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2018-03-22
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 32 2 1
Unit cell lengths	80.010, 80.010, 81.130
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.880 - 1.800
R-factor	0.156
Rwork	0.153
R-free	0.19600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xda per Morda
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MoRDa
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.880	35.880	1.850
High resolution limit [Å]	1.800	8.050	1.800
Rmerge	0.064	0.047	0.424
Rmeas	0.070	0.052	0.485
Number of reflections	28288	357	2076
<I/σ(I)>	15.7	27.07	2.9
Completeness [%]	99.9	96.2	99.8
Redundancy	5.857	4.852	4.083
CC(1/2)	0.998	0.999	0.863

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	285	Molecular Dimensions Morpheus screen, E9: 10% PEG 20.000, 20% PEG MME 550: 30mM of each diethyleneglycol, triethyleneglycol, tetraethyleneglycol, pentaethyleneglycol: 100mM Bicine/Trizma base pH 8.5: CrneC.01141.a.B12.PW38396 at 19.46mg/ml: cryo: direct: tray 297317e9: puck rdz7-2: