6CVN
Model of synthetic tau (R2x4) bound to the microtubule
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Phase Plate | VOLTA PHASE PLATE |
| Detector | GATAN K2 BASE (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.9 |
| Number of Particle | 24571 |
| Software | Appion,FREALIGN,Rosetta,SerialEM,CTFFIND,EMAN2 |
| CTF correction | PHASE FLIPPING ONLY |
