6CVD

High resolution crystal structure of FtsY-NG domain of E. coli bound to fragment 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953700
Spacegroup name	P 1 21 1
Unit cell lengths	34.341, 76.008, 108.032
Unit cell angles	90.00, 90.42, 90.00

Refinement procedure

Resolution	36.010 - 1.780
R-factor	0.2045
Rwork	0.202
R-free	0.25150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6cqp
RMSD bond length	0.012
RMSD bond angle	1.135
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.010	1.844
High resolution limit [Å]	1.780	1.780
Rmerge	0.031	0.322
Rmeas	0.044
Number of reflections	53304
<I/σ(I)>	17
Completeness [%]	99.8
Redundancy	2
CC(1/2)	0.998	0.814

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		297.15	0.1 M Bis-Tris pH 6.2, 25% PEG3350, 230 mM Ammonium acetate