6CVA

Crystal structure of the N. meningitides methionine-binding protein in its substrate-free conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97946
Spacegroup name	P 21 21 2
Unit cell lengths	52.564, 89.663, 45.088
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.223 - 1.559
R-factor	0.1902
Rwork	0.188
R-free	0.22010
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.681
Data reduction software	XDS
Data scaling software	Aimless (0.5.31)
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.223	34.223	1.220
High resolution limit [Å]	1.190	5.320	1.190
Rmerge	0.111	0.059	4.884
Rmeas	0.132	0.069	6.153
Total number of observations	425726
Number of reflections	128188	1512	7045
<I/σ(I)>	4.23	17.15	0.15
Completeness [%]	95.6	97.6	71
Redundancy	3.321	3.438	2.55
CC(1/2)	0.995	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2M MgCl2, 0.1M Bis-Tris pH5.5, 25% PEG3350