6CV9
Cytoplasmic domain of mTRPC6
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK III |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 0.001 |
Energy Filter | GIF Bioquantum |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.8 |
Number of Particle | 663034 |
Software | RELION,EPU,Gctf,Coot,PHENIX |
Symmetry | C4 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |