6CUQ
Crystal structure of Macrophage migration inhibitory factor-like protein (EhMIF) from Entamoeba histolytica
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-03-01 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97872 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 77.610, 100.060, 98.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.376 - 2.450 |
R-factor | 0.1822 |
Rwork | 0.177 |
R-free | 0.22530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3t5s selected by MorDa |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MoRDa |
Refinement software | PHENIX ((dev_3063)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 38.376 | 38.376 | 2.510 |
High resolution limit [Å] | 2.450 | 10.960 | 2.450 |
Rmerge | 0.032 | 0.023 | 0.547 |
Rmeas | 0.035 | 0.026 | 0.587 |
Number of reflections | 14405 | 183 | 1048 |
<I/σ(I)> | 32.96 | 70.09 | 4.2 |
Completeness [%] | 99.9 | 94.8 | 99.9 |
Redundancy | 7.349 | 5.475 | 7.498 |
CC(1/2) | 1.000 | 0.999 | 0.954 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 290 | Microlytics MCSG1 screen, condition G9: 20% PEG 3350, 200mM sodium formate: EnhiA.00834.a.B1.PS38385 at 22.83mg/ml: cryo: 20% EG: tray 297191g9: puck gmt2-2 |