6CUK
Engineered Cytochrome c from Rhodothermus marinus, Rma TDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.038, 60.038, 77.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.650 - 1.470 |
| R-factor | 0.18497 |
| Rwork | 0.184 |
| R-free | 0.20856 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cp5 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.719 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.650 | 1.500 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Number of reflections | 27920 | |
| <I/σ(I)> | 15.2 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.43-1.48 M (NH4)2SO4 300-400 mM NaSCN 1.5% isopropanol 14.0 mg/mL protein in 20 mM Tris pH 7.5 2:1 protein to well solution |
