6CU8
Alpha Synuclein fibril formed by full length protein - Twister Polymorph
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Quantum LS |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.6 |
| Number of Particle | 182253 |
| Software | EMAN,CNS,PHENIX,RELION,Leginon,CTFFIND |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
