6CT8
Selenomethionine structure of N-truncated R2-type pyocin tail fiber at 2.6 angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 120 |
Detector technology | PIXEL |
Collection date | 2013-12-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.980 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 59.930, 59.930, 396.633 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.983 - 2.620 |
R-factor | 0.1849 |
Rwork | 0.179 |
R-free | 0.23420 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.851 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (2.6.0) |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.670 |
High resolution limit [Å] | 2.620 | 7.110 | 2.620 |
Rmerge | 0.060 | 0.041 | 0.117 |
Rmeas | 0.064 | 0.044 | 0.128 |
Rpim | 0.021 | 0.015 | 0.049 |
Total number of observations | 103793 | ||
Number of reflections | 13424 | 860 | 633 |
<I/σ(I)> | 17.8 | ||
Completeness [%] | 96.5 | 97.6 | 97.1 |
Redundancy | 7.7 | 7.7 | 6 |
CC(1/2) | 0.999 | 0.993 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | Crystals were obtained at a 1:1 protein (6mg/mL) to precipitant (1M Na Acetate, 100mM CAPS, 100 mM LiOAc) |