6CT7
Fab of anti-a-synuclein antibody BIIB054 in complex with acetylated a-synuclein peptide (1-10)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-15 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.849, 101.492, 73.400 |
| Unit cell angles | 90.00, 117.30, 90.00 |
Refinement procedure
| Resolution | 24.860 - 1.903 |
| R-factor | 0.1819 |
| Rwork | 0.180 |
| R-free | 0.22290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.953 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.972 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.085 | |
| Number of reflections | 72048 | |
| <I/σ(I)> | 5.3 | |
| Completeness [%] | 97.0 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.9 | 277 | 18% w/v PEG 3350, 17% w/v PEG 400, 4.8% v/v 2-propanol, and 100 mM CAPSO pH 8.9 |
