6CSU

The structure of the Cep63-Cep152 heterotetrameric complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-04
Detector	DECTRIS PILATUS3 R CdTe 300K
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	28.066, 41.523, 205.619
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.870 - 2.500
R-factor	0.2535
Rwork	0.250
R-free	0.28530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.601
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.870	2.540
High resolution limit [Å]	2.500	2.500
Number of reflections	8163
<I/σ(I)>	16.68
Completeness [%]	91.2
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M MES (pH 5.4), 23% PEG 3350 and 0.2 M ammonium sulfate