6CSC
CHICKEN CITRATE SYNTHASE COMPLEX WITH TRIFLUOROACETONYL-COA AND CITRATE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Collection date | 1993-01-15 |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 110.370, 100.210, 87.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.250 |
| Rwork | 0.158 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1csc |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.300 |
| Data reduction software | SDMS ((DCREDUCE)) |
| Data scaling software | SDMS ((SCALE)) |
| Phasing software | FASTRAN |
| Refinement software | TNT (5F) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.400 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.051 | 0.138 |
| Number of reflections | 40589 | |
| <I/σ(I)> | 9.1 | 2.5 |
| Completeness [%] | 84.0 | 51 |
| Redundancy | 2.6 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | CRYSTALLIZED FROM 1.05M NA CITRATE, PH 6.0 2 MM TRIFLUOROACETONYL COENZYME A |
