6CSC

CHICKEN CITRATE SYNTHASE COMPLEX WITH TRIFLUOROACETONYL-COA AND CITRATE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	295
Detector technology	AREA DETECTOR
Collection date	1993-01-15
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 21 21 21
Unit cell lengths	110.370, 100.210, 87.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.250
Rwork	0.158
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1csc
RMSD bond length	0.014
RMSD bond angle	2.300
Data reduction software	SDMS ((DCREDUCE))
Data scaling software	SDMS ((SCALE))
Phasing software	FASTRAN
Refinement software	TNT (5F)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.400
High resolution limit [Å]	2.230	2.230
Rmerge	0.051	0.138
Number of reflections	40589
<I/σ(I)>	9.1	2.5
Completeness [%]	84.0	51
Redundancy	2.6	1.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		CRYSTALLIZED FROM 1.05M NA CITRATE, PH 6.0 2 MM TRIFLUOROACETONYL COENZYME A