6CS8

High resolution crystal structure of FtsY-NG domain of E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953700
Spacegroup name	P 1 21 1
Unit cell lengths	34.541, 76.192, 107.978
Unit cell angles	90.00, 90.72, 90.00

Refinement procedure

Resolution	35.990 - 1.749
R-factor	0.1823
Rwork	0.180
R-free	0.22050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.031
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.990	1.812
High resolution limit [Å]	1.749	1.749
Rmerge	0.027	0.409
Rmeas	0.038
Number of reflections	56278
<I/σ(I)>	19.23
Completeness [%]	99.5
Redundancy	2
CC(1/2)	0.999	0.716

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		297.15	0.1 M Bis-Tris pH 6.6, 22% PEG3350, 185 mM Ammonium Acetate