6CRC
Ternary complex crystal structure of DNA polymerase Beta with a dideoxy terminated primer with CCL2, beta, gamma dATP analogue
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-05-12 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.437, 78.939, 54.911 |
Unit cell angles | 90.00, 108.55, 90.00 |
Refinement procedure
Resolution | 19.980 - 2.300 |
R-factor | 0.2144 |
Rwork | 0.206 |
R-free | 0.28500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fms |
RMSD bond length | 0.009 |
RMSD bond angle | 1.152 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 2.340 |
High resolution limit [Å] | 2.300 | 6.180 | 2.300 |
Rmerge | 0.094 | 0.035 | 0.471 |
Total number of observations | 69008 | ||
Number of reflections | 19268 | 1021 | 808 |
<I/σ(I)> | 6.8 | ||
Completeness [%] | 97.9 | 100 | 83.2 |
Redundancy | 3.6 | 3.7 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 16-18% PEG 3350 |