6CQP

High resolution crystal structure of FtsY-NG domain of E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-05-19
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.953724980354
Spacegroup name	P 1 21 1
Unit cell lengths	34.697, 76.105, 108.871
Unit cell angles	90.00, 93.64, 90.00

Refinement procedure

Resolution	44.216 - 1.446
R-factor	0.1342
Rwork	0.132
R-free	0.17720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fts
RMSD bond length	0.003
RMSD bond angle	0.611
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.050	1.498
High resolution limit [Å]	1.446	1.446
Rmerge	0.021	0.089
Number of reflections	99964
<I/σ(I)>	20.69
Completeness [%]	99.2
Redundancy	2	2.73
CC(1/2)	0.999	0.979

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	297.15	0.1 M Bis-Tris pH 6.2, 28% PEG3350, 165mM NaCl