6CQM
Crystal Structure of mitochondrial single-stranded DNA binding proteins from S. cerevisiae, Rim1 (Form2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2016-01-27 |
Detector | RDI CMOS_8M |
Wavelength(s) | 0.987 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 82.470, 103.540, 114.580 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.128 - 3.000 |
R-factor | 0.2407 |
Rwork | 0.238 |
R-free | 0.29410 |
Structure solution method | SAD |
RMSD bond length | 0.001 |
RMSD bond angle | 0.372 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.140 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.035 | 0.231 |
Rpim | 0.035 | 0.231 |
Number of reflections | 24765 | 1977 |
<I/σ(I)> | 19 | |
Completeness [%] | 99.8 | |
Redundancy | 13.9 | |
CC(1/2) | 0.998 | 0.867 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 295 | 0.2 M MgCl2 and 20% (w/v) PEG 3350 |