6CQF
Crystal structure of HPK1 in complex an inhibitor G1858
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2014-03-12 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97872 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 91.868, 98.803, 77.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.639 - 2.246 |
R-factor | 0.2101 |
Rwork | 0.208 |
R-free | 0.25510 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.954 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 16438 | |
<I/σ(I)> | 27.4 | |
Completeness [%] | 96.4 | |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 0.1 M Tris-HCl, pH 8.5, 0.25 M sodium tartrate, 12% PEG8000 |