6CPY

Structure of apo GRMZM2G135359 pseudokinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-28
Detector	DECTRIS EIGER 16M
Wavelength(s)	0.979180
Spacegroup name	P 21 21 21
Unit cell lengths	61.492, 61.496, 144.764
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.820 - 1.700
R-factor	0.20021
Rwork	0.199
R-free	0.21529
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5uv4
RMSD bond length	0.007
RMSD bond angle	1.102
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0218)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.820	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.064	2.035
Rmeas	0.070	2.247
Rpim	0.029	0.943
Number of reflections	61213
<I/σ(I)>	16.4
Completeness [%]	99.9	99.9
Redundancy	10.88
CC(1/2)		0.519

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.15 M potassium bromide, 30% PEG2000 MME