6CPQ
Structure of human DNA polymerase beta complexed with 8-ClG in the template base paired with incoming non-hydrolyzable CTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97648 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.829, 80.243, 55.836 |
Unit cell angles | 90.00, 107.80, 90.00 |
Refinement procedure
Resolution | 19.854 - 1.930 |
R-factor | 0.1935 |
Rwork | 0.191 |
R-free | 0.23880 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.183 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.960 |
High resolution limit [Å] | 1.930 | 1.930 |
Number of reflections | 32119 | |
<I/σ(I)> | 31.2 | |
Completeness [%] | 100.0 | |
Redundancy | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 14% to 23% PEG3400, and 350 mM sodium acetate in 50 mM imidazole (pH 7.5) |