6CPO
Crystal structure of DR15 presenting the RQ13 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-09 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.740, 73.500, 94.940 |
Unit cell angles | 90.00, 106.95, 90.00 |
Refinement procedure
Resolution | 47.080 - 2.400 |
R-factor | 0.205 |
Rwork | 0.203 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mdo |
RMSD bond length | 0.007 |
RMSD bond angle | 0.990 |
Data reduction software | XDS |
Data scaling software | Aimless |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.200 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rpim | 0.076 | 0.408 |
Number of reflections | 34562 | 3604 |
<I/σ(I)> | 9 | |
Completeness [%] | 100.0 | |
Redundancy | 9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | PBS pH 7.5, Na acetate, PEG 4000 |