6CP8
Contact-dependent growth inhibition toxin-immunity protein complex from from E. coli 3006
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.707, 106.534, 72.654 |
| Unit cell angles | 90.00, 101.01, 90.00 |
Refinement procedure
| Resolution | 29.632 - 2.201 |
| R-factor | 0.1774 |
| Rwork | 0.176 |
| R-free | 0.21810 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.823 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.127 | 0.749 |
| Number of reflections | 38085 | 1879 |
| <I/σ(I)> | 11.28 | 1.96 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.2 | 3.8 |
| CC(1/2) | 0.664 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1 M HEPES/Na pH 7.0, 18% PEG12K |
