6CP0
SdcA in complex with the E2, UbcH5C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2014-11-22 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.6309 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 135.550, 142.203, 118.338 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.010 |
| R-factor | 0.19746 |
| Rwork | 0.194 |
| R-free | 0.26053 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4trg |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.468 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 25193 | |
| <I/σ(I)> | 8.8 | |
| Completeness [%] | 96.4 | |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.5 M ammonium sulfate, 3% glycerol, 0.1 M HEPES pH 7.5 |
