6COK
Structure of the 2nd TOG domain from yeast CLASP protein STU1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.412, 111.009, 44.937 |
| Unit cell angles | 90.00, 101.36, 90.00 |
Refinement procedure
| Resolution | 40.949 - 1.890 |
| R-factor | 0.1747 |
| Rwork | 0.173 |
| R-free | 0.21210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.810 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.074 | 0.568 |
| Rpim | 0.040 | 0.330 |
| Number of reflections | 23899 | |
| <I/σ(I)> | 16.7 | |
| Completeness [%] | 98.6 | |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 18% PEG3350, 0.1 M HEPES pH 7.5 |
