6CO6
Crystal structure of Rhodococcus jostii RHA1 IpdAB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-12 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97948 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 68.910, 68.910, 241.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.727 - 1.701 |
| R-factor | 0.1659 |
| Rwork | 0.165 |
| R-free | 0.19230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1poi |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.850 | 1.762 |
| High resolution limit [Å] | 1.701 | 1.701 |
| Rmerge | 0.091 | |
| Number of reflections | 65095 | 6374 |
| <I/σ(I)> | 16.54 | 2.31 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8 | 8 |
| CC(1/2) | 0.818 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.9 M ammonium sulfate, 0.2 M sodium potassium tartrate |
