6CO6

Crystal structure of Rhodococcus jostii RHA1 IpdAB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-12
Detector	RAYONIX MX300HE
Wavelength(s)	0.97948
Spacegroup name	P 43 21 2
Unit cell lengths	68.910, 68.910, 241.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.727 - 1.701
R-factor	0.1659
Rwork	0.165
R-free	0.19230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1poi
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.850	1.762
High resolution limit [Å]	1.701	1.701
Rmerge	0.091
Number of reflections	65095	6374
<I/σ(I)>	16.54	2.31
Completeness [%]	100.0	100
Redundancy	8	8
CC(1/2)		0.818

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.9 M ammonium sulfate, 0.2 M sodium potassium tartrate