Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2006-01-01 |
Detector | APEX II CCD |
Wavelength(s) | 0.97937 |
Spacegroup name | P 1 |
Unit cell lengths | 38.308, 57.767, 65.657 |
Unit cell angles | 91.32, 89.99, 90.88 |
Refinement procedure
Resolution | 19.000 - 1.800 |
R-factor | 0.16364 |
Rwork | 0.161 |
R-free | 0.21125 |
Structure solution method | SAD |
RMSD bond length | 0.020 |
RMSD bond angle | 1.965 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.077 | 0.144 |
Number of reflections | 50614 | 49555 |
<I/σ(I)> | 27.9 | |
Completeness [%] | 97.6 | 96.9 |
Redundancy | 4.3 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 24% PEG2000 MME, 300 mM sodium chloride, 100 mM HEPES, pH 7.5 |