6CMG

Crystal Structure of the Hendra Virus Attachment G Glycoprotein Bound to a Potent Cross-Reactive Neutralizing Human Monoclonal Antibody m102.3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-03-01
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 61 2 2
Unit cell lengths	72.916, 72.916, 653.814
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.421 - 2.700
R-factor	0.2316
Rwork	0.229
R-free	0.27470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.860
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.700	7.320	2.700
Rmerge	0.125	0.047	0.714
Total number of observations	223509
Number of reflections	30353	1806	1439
<I/σ(I)>	5.9
Completeness [%]	99.9	98.3	100
Redundancy	7.4	6.1	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	17% PEG 3350, 0.18M (NH4)3 Citrate