6CMC
Barium sites in the structure of a desensitized acid sensing ion channel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.93725 |
Spacegroup name | H 3 |
Unit cell lengths | 133.938, 133.938, 122.671 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.140 - 3.671 |
R-factor | 0.2842 |
Rwork | 0.283 |
R-free | 0.29950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nyk |
RMSD bond length | 0.004 |
RMSD bond angle | 0.978 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((dev_2597: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.140 | 3.800 |
High resolution limit [Å] | 3.670 | 3.670 |
Number of reflections | 8949 | |
<I/σ(I)> | 6.78 | |
Completeness [%] | 99.6 | |
Redundancy | 8.12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 293.15 | 100 mM HEPES pH 6.9, 150 mM sodium formate, 18% (w/v) PEG 3,350. Crystal soaked in 50 mM BaCl2 prior to freezing |