6CLX
Crystal structure of TnmH in complex with SAM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-12-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 116.220, 116.220, 110.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.240 - 2.730 |
| R-factor | 0.22793 |
| Rwork | 0.225 |
| R-free | 0.27780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6clw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.354 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.240 | 2.880 |
| High resolution limit [Å] | 2.730 | 2.730 |
| Rmerge | 0.148 | 0.871 |
| Rpim | 0.088 | 0.554 |
| Number of reflections | 23384 | 3362 |
| <I/σ(I)> | 10.4 | |
| Completeness [%] | 100.0 | |
| Redundancy | 7 | |
| CC(1/2) | 0.995 | 0.580 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M L-Proline, 0.1 M HEPES, pH 7.5, 10% w/v Polyethlene glycol 3,350 |
