6CLW
Crystal structure of TnmH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-09-27 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 110.860, 110.860, 113.310 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.660 - 2.740 |
| R-factor | 0.20501 |
| Rwork | 0.202 |
| R-free | 0.25436 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3i53 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.385 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.660 | 2.890 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Number of reflections | 21574 | |
| <I/σ(I)> | 10 | |
| Completeness [%] | 99.9 | |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M L-Proline, 0.1 M HEPES, pH 7.5, 10% w/v Polyethylene glycol 3,350 |
