6CL4
LipC12 - Lipase from metagenomics
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-07-29 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 58.500, 58.500, 192.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.175 - 2.640 |
R-factor | 0.195 |
Rwork | 0.192 |
R-free | 0.25100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ex9 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.447 |
Data reduction software | XDS (VERSION December 28, 2009) |
Data scaling software | XSCALE (VERSION January 30, 2009) |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.500 | 2.720 |
High resolution limit [Å] | 2.640 | 2.640 |
Rmeas | 0.140 | 0.641 |
Number of reflections | 10489 | 851 |
<I/σ(I)> | 18.91 | 4.29 |
Completeness [%] | 95.5 | 97.7 |
Redundancy | 8.82 | 8.38 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 2.0 M sodium formate and 0.1 M bis-tris propane, pH 7.0 using a protein concentration of 10 mg / mL. Crystals grown for ten weeks |