6CKG

D-glycerate 3-kinase from Cryptococcus neoformans var. grubii serotype A (H99 / ATCC 208821 / CBS 10515 / FGSC 9487)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-26
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	85.740, 87.760, 103.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.520 - 2.000
R-factor	0.1805
Rwork	0.179
R-free	0.22510
RMSD bond length	0.007
RMSD bond angle	0.818
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MoRDa
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	53013
<I/σ(I)>	15.78
Completeness [%]	99.6
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.4 uL 20 mg/mL JCSG+ B4, CrneC.19325.a.B1.PW38244 + 0.4 uL 0.1 M HEPES, pH 7.5, 10% w/v PEG8000, 8% v/v ethylene glycol, cryoprotectant: 20% ethylene glycol