6CK9
Crystal Structure of HIV-1 ConC_Base0 Prefusion Env Trimer in Complex with Human Antibody Fragment 3H109L and 35O22 variants at 3.5 Angstrom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2017-08-22 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1 |
Spacegroup name | P 63 |
Unit cell lengths | 131.900, 131.900, 314.860 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.174 - 2.714 |
R-factor | 0.2504 |
Rwork | 0.248 |
R-free | 0.28760 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.760 |
High resolution limit [Å] | 2.710 | 7.350 | 2.710 |
Rmerge | 0.108 | 0.057 | 1.693 |
Rmeas | 0.126 | 0.067 | 2.035 |
Rpim | 0.064 | 0.034 | 1.095 |
Total number of observations | 153622 | ||
Number of reflections | 29641 | 4222 | 290 |
<I/σ(I)> | 5.7 | ||
Completeness [%] | 53.5 | 99.2 | 6.9 |
Redundancy | 3.4 | 3.7 | 2.3 |
CC(1/2) | 0.990 | 0.221 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 75mM HEPES pH7.5; 2% PEG 8,000 and 10% PEG 400 |