6CK4
G96A mutant of the PRPP riboswitch from T. mathranii bound to ppGpp
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-10 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 |
Unit cell lengths | 53.252, 62.147, 125.909 |
Unit cell angles | 91.18, 89.61, 101.94 |
Refinement procedure
Resolution | 36.539 - 3.097 |
R-factor | 0.2509 |
Rwork | 0.248 |
R-free | 0.30410 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.018 |
RMSD bond angle | 1.852 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.7.17) |
Refinement software | PHENIX (1.12-2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 3.150 |
High resolution limit [Å] | 3.097 | 8.390 | 3.100 |
Rmerge | 0.099 | 0.032 | 1.222 |
Rmeas | 0.130 | 0.042 | 1.604 |
Rpim | 0.082 | 0.026 | 1.026 |
Number of reflections | 26741 | 1332 | 1309 |
<I/σ(I)> | 3.9 | ||
Completeness [%] | 93.7 | 93.2 | 94.7 |
Redundancy | 2.2 | 2.3 | 2.1 |
CC(1/2) | 0.992 | 0.253 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7.5 | 296.15 | 1 uL 150 uM RNA in 10 mM magnesium chloride, 10 mM potassium chloride, 10 mM HEPES-KOH, pH 7.5, 1 mM ppGpp, mixed with 0.8 uL 80 mM sodium chloride, 40 mM sodium cacodylate, pH 7.0, 30% MPD, 12 mM spermine |