6CJO

Crystal Structure of Chalcone Isomerase from Medicago Sativa with the G95S mutation.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00000
Spacegroup name	P 65 2 2
Unit cell lengths	90.395, 90.395, 351.775
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.928 - 2.400
R-factor	0.2325
Rwork	0.231
R-free	0.25270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.749
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.940	2.460
High resolution limit [Å]	2.400	2.400
Number of reflections	34433
<I/σ(I)>	11.1
Completeness [%]	99.9
Redundancy	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	20mgs/ml in 2.2M AmSO4, 50mM hepes pH 7.5 and 25% glycerol