6CJC
CRYSTAL STRUCTURE OF A FC FRAGMENT LALA MUTANT (L234A, L235A) OF HUMAN IGG1 (CRYSTAL FORM 3)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-31 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.962, 75.188, 148.295 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.530 - 2.575 |
R-factor | 0.195 |
Rwork | 0.193 |
R-free | 0.24060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ave |
RMSD bond length | 0.005 |
RMSD bond angle | 1.008 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.575 | 2.575 |
Rmerge | 0.232 | |
Rpim | 0.100 | |
Number of reflections | 17739 | 845 |
<I/σ(I)> | 15.3 | 1.3 |
Completeness [%] | 99.7 | 100 |
Redundancy | 6.3 | |
CC(1/2) | 0.274 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 20% PEG MME 5,000 0.1 M Bis-Tris. pH 6.5 |