6CIQ
Pyruvate:ferredoxin oxidoreductase from Moorella thermoacetica with coenzyme A bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9789 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 337.710, 106.993, 120.465 |
Unit cell angles | 90.00, 109.85, 90.00 |
Refinement procedure
Resolution | 80.252 - 3.302 |
R-factor | 0.1858 |
Rwork | 0.184 |
R-free | 0.22270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6cin |
RMSD bond length | 0.003 |
RMSD bond angle | 0.566 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 3.360 |
High resolution limit [Å] | 3.300 | 3.300 |
Number of reflections | 58817 | 2499 |
<I/σ(I)> | 3.5 | |
Completeness [%] | 97.3 | |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 13% (w/v) PEG 6000, 0.06 M sodium cacodylate pH 6.5 |