6CII

Crystal structure of Chlamydomonas reinhardtii THB1 in the cyanomet state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OTHER
Temperature [K]	110
Detector technology	CCD
Collection date	2017-11-10
Detector	AGILENT ATLAS CCD
Wavelength(s)	1.54
Spacegroup name	P 41 2 2
Unit cell lengths	45.992, 45.992, 121.277
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.502 - 1.700
R-factor	0.166
Rwork	0.163
R-free	0.19050
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.103
Data reduction software	CrysalisPro (1.171.36.32)
Data scaling software	CrysalisPro (1.171.36.32)
Phasing software	PHENIX (1.13rc1_2954)
Refinement software	PHENIX ((1.13rc1_2954: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.502	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.042	0.192
Rmeas	0.044	0.214
Rpim	0.013	0.093
Number of reflections	27344	2732
<I/σ(I)>	36.06	5.88
Completeness [%]	100.0	99.9
Redundancy	8.2	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	0.1 M Bis-Tris pH 5.5, 30% PEG 3350